Identification of DVA Interneuron Regulatory Sequences in Caenorhabditis elegans
Figure 7
A. Mutation of WT24 in the context of larger fragments. The sequences of WT53 and sub-fragments WT29 and WT24 are shown in black type. WT53 is the black box in the diagrams of WT195 and WT85. Mutations of the bases of WT24 are in red type in Mut24. Mut24 is shown as a red box in the diagrams of Mut195 and Mut85. Neuronal expression in transgenic lines derived from the four experimental sequences is shown in the box as either+or – expression under DVA or Broad. B. Mutation of the flanking sequences of WT53. The WT53 sequence is shown in black type at top. WT53 sequence is represented by the black box with flanking wild-type sequences found in WT85 in black type. Mutations of the flanking sequences are shown in red type and labeled: 5′ Mut85 (mutation of the 17′bp 5′ of WT53); 3′ Mut85 (mutation of the 14 bp 3′ of WT53); and 5′3′ Mut85 (mutation of the 5′ and 3′ flanking sequences). Expression in DVA or Broad neuronal expression is denoted as+or - in the box to the right of the experimental sequences.