Differential Effects of Serum Heat Treatment on Chemotaxis and Phagocytosis by Human Neutrophils
Figure 1
Example histograms of the fluorescence intensity of stained zymosan particles measured by flow cytometry.
(A) Results of staining with FITC-conjugated anti-C3b. (B) Results of staining with Alexa-Fluor®-488-conjugated anti-IgG. In all cases, the particles had been incubated in three different buffers, i.e., serum-free HBSS (“plain”), HBSS with 10% untreated autologous serum (37°C-ASPO), and HBSS with 10% autologous serum that had been heat-treated at 56°C (56°C-ASPO). The legend included in (A) applies to all panels.