Immunopositivity for Histone MacroH2A1 Isoforms Marks Steatosis-Associated Hepatocellular Carcinoma
Figure 2
Protein expression of macroH2A1 isoforms in the liver of the liver-specific PTEN KO mouse model of steatosis and HCC.
A. Representative pictures of trichrome staining in liver samples from PTENflox/flox and PTEN KO mice. B. histone extracts from livers of 16 weeks old PTENflox/flox, 16 weeks old PTEN KO and from liver tumors of 52 weeks old PTEN KO mice were processed for immunoblotting. MacroH2A1.1, macroH2A1.2 and histone H3 (loading control) protein levels were detected with specific antibodies. Representative immunoblots are shown. C. Densitometric quantification of macroH2A1.1, macroH2A1.2 protein levels in the livers of 4 months old PTENflox/flox (N = 5), PTEN KO 16 weeks old (N = 5) and in the liver tumors of PTEN KO 52 weeks old mice (N = 2), * P<0.05, ** P<0.01 versus PTENflox/flox mice.