MicroRNA-22 (miR-22) Overexpression Is Neuroprotective via General Anti-Apoptotic Effects and May also Target Specific Huntington’s Disease-Related Mechanisms
Figure 3
miR-22 decreases the focal accumulation of Htt protein.
Primary striatal neurons were infected on DIV1 with lentiviral vectors encoding tTA and mutant (Htt82Q) Htt171 fragments under the control of the TRE promoter. Where indicated cells were co-infected on DIV4 with vector encoding miR-22. Htt accumulation was assessed using anti-Htt antibody 2B4. A) Numerous Htt-positive foci were detected in cells expressing Htt171-82Q. B) Fewer Htt-positive foci were detected in cells expressing miR-22+ Htt171-82Q. C) Htt-enriched foci were quantified using ImageJ software as described in Methods. Graphs represent mean ± SEM, n = 10. ** represents p-value <0.01, by unpaired t-test. Scale bar = 10 µm.