Profilin 1 as a Target for Cathepsin X Activity in Tumor Cells
Figure 4
Co-localization of cathepsin X and profilin 1 (A) with clathrin (B) and actin (C).
All proteins were visualized by immunofluorescence staining using primary antibodies to cathepsin X, profilin 1 and clathrin, followed by Alexa Fluor conjugated secondary antibodies Alexa Fluor 488, 555 and 633 or phalloidin conjugate for actin. (A) Cathepsin X is shown in green, profilin 1 in red and co-localization in yellow. For co-localization, also Zen 2011 Software (Carl Zeiss) option for improved visibility of co-localized pixels was used and co-localization is shown in white with corresponding scatter diagram. (B) Clathrin is shown in red, profilin 1 and cathepsin X are both in green due to clearer merged image. (C) Profilin 1 is shown in green, cathepsin X in blue and actin in red. Zen 2011 Software option for improved visibility of co-localization is used. Bars, 10 µm.