Ammonium Accumulation and Cell Death in a Rat 3D Brain Cell Model of Glutaric Aciduria Type I
Figure 2
Effects of GA and 3-OHGA on neurons.
(Left panel) Immunohistochemical staining for phosphorylated medium weight neurofilament (p-NFM) on cryosections of cultures derived from protocol A (DIV 8) and protocol B (DIV 14). Scale bar: 100 µm. (Right panel) Representative western blots with data quantification of whole-cell lysates for p-NFM for protocol A (DIV 8, above) and protocol B (DIV 14, below). Actin was used as a loading control. The quantifications of p-NFM are expressed as percentage of respective controls. The values represent the mean ± SD from 3 replicates taken from 2 independent experiments.