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The 1.6 Å Crystal Structure of Pyranose Dehydrogenase from Agaricus meleagris Rationalizes Substrate Specificity and Reveals a Flavin Intermediate

Figure 4

Absorption spectra of reduced AmPDH reacting with molecular oxygen.

Oxidation of PDH by oxygen at air-saturation as a function of time at 25°C. Line 1: reduced PDH at start of the reaction which lacks the flavin absorption shoulder around 490 nm; line 5, the spectrum of partially oxidized PDH (∼40% oxidation); line 10, the absorption spectrum of partially oxidized PDH after the reaction proceeded for 15 h.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0053567.g004