Integration of Transcriptome, Proteome and Metabolism Data Reveals the Alkaloids Biosynthesis in Macleaya cordata and Macleaya microcarpa
Figure 7
Quantitative RT-PCR validataion.
Four candidate unigenes (BBE, P6H, SAR and TNMT) are shown for involved in the BIAs metabolic pathway show differential expression patterns by RT-PCR in three organs were carried out on cDNA prepared from roots (phases I and II), leaves (phases I and II), and fruits shells (phase II) as described in the Material and Methods (phase I show in blue bars and phase II show in red bars). Expression level is relative to ubiquitin and all results represent the mean (± SD) of three experiments.