Histone Deacetylase Inhibition Decreases Cholesterol Levels in Neuronal Cells by Modulating Key Genes in Cholesterol Synthesis, Uptake and Efflux
Figure 6
TSA treatment partially reverts the effect of U18666A on the expression of genes involved in cholesterol synthesis, uptake and efflux.
SH-SY5Y neuroblastoma cells were pre-treated with 3 µg/ml U18666A for 6 h and with or without 250 nM TSA for 16 h. mRNA transcript levels of HMGCR, MVK, LDLR and ABCA1 were analyzed by qPCR. Values were normalized to the internal standard β-actin and are expressed as fold change relative to untreated cells. Data represent means ± SEM from at least three individual experiments (* p<0.05, ** p<0.01, *** p<0.001).