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Overexpression of the AtSHI Gene in Poinsettia, Euphorbia pulcherrima, Results in Compact Plants

Figure 4

Quantitative real-time PCR analysis of AtSHI transgene in different transgenic lines of poinsettia.

Two microgram total RNAs from transgenic poinsettia lines and the endogenous control, α-tubulin gene were used for synthesize cDNAs prior the real-time qPCR analysis. Values are means of three technical replications except TL2 (n = 2). Data were analyzed using the 2−△△CT method and represented as log10 values. Vertical bars represent the ± SE (standard error).

Figure 4

doi: https://doi.org/10.1371/journal.pone.0053377.g004