Role of CEACAM1 and CEACAM20 in an In Vitro Model of Prostate Morphogenesis
Figure 5
Phenotype analysis of hPrECs grown on-Matrigel.
(A) PCR analysis for CK8, CK18, CK5, CK14, AR, PSA, PAP, CD133 and PSCA. (B) Western blot analysis for CK18, CK5, CK14 and PAP. (C) FACS analysis for CK8/18, AR, PAP and PSA, (D) Confocal microscopy analysis of organoids on-Matrigel stained with anti-AR (green) or anti-PSA (green) or anti-PAP (green), phalloidin (red) and DAPI (blue), scale bar 50 µm.