A Novel Immunofluorescent Computed Tomography (ICT) Method to Localise and Quantify Multiple Antigens in Large Tissue Volumes at High Resolution
Figure 2
Confocal microscopy three-dimensional reconstruction of a 2 µm thick BMMA tissue section from the mouse meibomian gland stained with anti-CK6 (red) and DAPI (blue).
Antibody penetration is observed throughout the entire 2 µm section for anti-CK6 and DAPI. Scale bar = 10 µm.