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A Novel Muscarinic Antagonist R2HBJJ Inhibits Non-Small Cell Lung Cancer Cell Growth and Arrests the Cell Cycle in G0/G1

Figure 4

mRNA expressions of cholinergic receptors and choline acetyltransferase in NSCLC cell lines.

RT-PCR was performed on total RNA prepared from the indicated cell lines. Primers were described in Table 3. GAPDH was used as loading control.

doi: https://doi.org/10.1371/journal.pone.0053170.g004