Amelogenesis Imperfecta in Two Families with Defined AMELX Deletions in ARHGAP6
Figure 9
Alternative ARHGAP6 promoter usage in secretory and maturation ameloblasts.
A: Map of the 5′ end of ARHGAP6 inclusive of the four promoters (indicated by exons 1a through 1d). Arrows indicate primer annealing sites for RT-PCR. B: PCR amplifcation products Key to RNA sources: Lanes 1: laser captured day 5 ameloblasts; Lanes 2: laser captured day 12 ameloblasts; Lanes 3: day 5 enamel organ epithelia; Lanes 4: day 12 enamel organ epithelia; Lanes 5: Positive PCR controls, spleen for 1a, lung for 1b, 1c, and 1d). These results indicate that Arhgap6 is expressed almost exclusively from promoter 1b in developing molars, and mostly during the maturation stage.