Monitoring Hypoxia Induced Changes in Cochlear Blood Flow and Hemoglobin Concentration Using a Combined Dual-Wavelength Laser Speckle Contrast Imaging and Doppler Optical Microangiography System
Figure 1
DWLSCI and DOMAG system setup.
(a) Synchronized dual wavelength laser speckle contrast imaging system setup. C = camera, DBS = dichroic beam splitter, EL = expanding lens, LD = laser diodes, M = mirror, N2 = nitrogen, O2 = oxygen, ZL = zoom lens. (b) Doppler optical microangiography system setup. CL = collimator, OC = optical coupler, OL = objective lens, PC = polarization controller, SLD = superluminescent diode. (c) Ventral view image of the cochlea after opening the bulla. The regions of the apical (A) and basal (B) turns, the stapedial artery (S) and the round window (RW) can be observed. (d) Mean and standard deviation of the systemic oxygen saturation of the five mice, indicating the hypoxia between minutes 3 and 7.