Cryopreserved Dental Pulp Tissues of Exfoliated Deciduous Teeth Is a Feasible Stem Cell Resource for Regenerative Medicine
Figure 2
(A–C) Dentinogenic/osteogenic differentiation capacity. Images of Alizarin Red staining (A) and alkaline phosphatase (ALP) activity (B) of SHED-Cryo. Comparison of Alizarin Red-positive (Alizarin Red+) area (A), ALP activity (B) and odontoblast/osteoblast-specific genes, runt-related gene 2 (RUNX2), ALP, osteocalcin (OCN), and dentin sialophosphoprotein (DSPP) (C). (D) Chondrogenic differentiation capacity. Comparison of chondrocyte-specific genes, SOX9, aggrecan (AGG) and type X collagen (ColX). (E, F) Adipogenic differentiation assay. A representative image of Oil Red-O staining and comparison of Oil Red-O accumulation (E). Comparison of adipocyte-specific genes lipoprotein lipase (LPL) and peroxisome proliferator activated receptor-gamma2 (PPARgamma2) (F). (G) Hepatogenic differentiation capacity. Comparison of hepatocyte-specific gene albumin (ALB). (H) Endothelial cell differentiation assay. Comparison of endothelial cell markers CD31 and CD34. (H) Neural cell differentiation assay. Comparison of neural cell markers neurofilament M (NFM) and tubulin betaIII (betaIII). A–I: n = 5 for all group. ns: no significance. The graph bars represent mean±SD.