Most Anti-BrdU Antibodies React with 2′-Deoxy-5-Ethynyluridine — The Method for the Effective Suppression of This Cross-Reactivity
Figure 7
The simultaneous localisation of BrdU and EdU.
The results of simultaneous localisation of BrdU and EdU using two different protocols for BrdU revelation in cells labelled for 5 minutes with BrdU and then after 13 hours for 20 minutes with EdU. A. The revelation of BrdU by 4N HCl is shown in the picture. The upper set of images represents the detection of both signals without the application of the blocking step by means of 2 mM azidomethylphenylsulfide. The bottom set of images represents the detection of both signals with the application of the blocking step by means of 2 mM azidomethylphenylsulfide. Barr: 20 µm. B. The revelation of BrdU by copper(I) ions and exonuclease III is shown in the picture. The upper set of images represents the detection of both signals without the application of the blocking step by means of 2 mM azidomethylphenylsulfide. The bottom set of images represents the detection of both signals with the application of the blocking step by means of 2 mM azidomethylphenylsulfide. Barr: 20 µm.