Microarray-Assisted Pathway Analysis Identifies MT1X & NFκB as Mediators of TCRP1-Associated Resistance to Cisplatin in Oral Squamous Cell Carcinoma
Figure 1
In vitro effects of TCRP1 interfering RNA on human OSCC.
A. Real-time PCR analysis of TCRP1 mRNA expression. Treatment of Tca/PYM cells with TCRP1 interfering plasmid (pAU-siTCRP1) led to a significant decrease in TCRP1 mRNA levels. No reduction in expression was observed for untreated cells or cells treated with scrambled interfering plasmid. B. TCRP1 expression in Tca/PYM, Tca/PYM-Con, and Tca/PYM-siTCRP1 cells were examined by Western blot. Decreased expression of TCRP1 was observed after transfection with TCRP1 interfering plasmid in Tca/PYM cells. No reduction in expression was observed for untreated cells or cells treated with scrambled interfering plasmid. β-actin was used as the loading control. C. Responses of Tca/PYM and Tca/PYM-siTCRP1 cells to PYM. Tca8113/PYM cells were more resistant to PYM. D. Responses of Tca/PYM and Tca/PYM-siTCRP1 cells to DDP. Tca8113/PYM-siTCRP1 cells were more sensitive to DDP.