Rhesus Macaque Theta Defensins Suppress Inflammatory Cytokines and Enhance Survival in Mouse Models of Bacteremic Sepsis
Figure 2
RTD-1 inhibits the release of soluble TNF in whole blood.
Human EDTA-anti-coagulated blood diluted 1∶10 in RPMI, was stimulated for 4 h with live E. coli cells (•), S. aureus 502a (⧫), or E. coli K12 LPS (□) with simultaneous addition of RTD-1 at the concentrations indicated. E. coli-stimulated blood was similarly analyzed for effects of a natural mixture of HNP 1–3 (▾). Blood incubated for 4 h with RTD-1 alone shown as (▵). N = 2–7 for each experiment.