Interferon-γ Upregulates Expression of IFP35 Gene in HeLa Cells via Interferon Regulatory Factor-1
Figure 7
STAT1 is involved in IFN-γ-induced IFP35 expression in HeLa cells.
(A) pGL-210 was co-transfected with shSTAT1 or shCONr into HeLa cells. At 36 h after transfection, cells were cultured in the presence or absence of IFN-γ (10 ng/ml) for 12 h before luciferase assays were performed. The response to IFN-γ is presented as fold induction relative to unstimulated cells. Data are the mean and standard error from three experiments. *P<0.05. (B) HeLa cells stably expressing either shSTAT1 or shCONr were transfected with pCDNA3.1 or pCDNA3.1-IRF-1. At 36 h after transfection, cells were treated with IFN-γ (10 ng/ml) for 12 h before immunoblotting was performed. (C) HeLa cells were treated with IFN-γ (10 ng/ml) for 12 h and processed for ChIP assays by using control IgG, anti-IRF-1 or anti-STAT1. The negative control indicates a genomic fragment (+976 to +1337, relative to the translation start site) downstream of IL-7 promoter.