Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

The Dorsolateral Periaqueductal Gray and Its Role in Mediating Fear Learning to Life Threatening Events

Figure 8

Experiment 5– Effects of dorsal premammillary nucleus blockade on dlPAG-NMDA OFC acquisition.

The experimental design used is outlined above the graph, where vertical arrows show when the animals received the PMd and dlPAG infusions and were placed in a conditioning box with the olfactory CS. Histograms represent the mean (+SEM) of the percentage of approach time (A), hide time (B), and head-out time (C). The hatched horizontal line represents the mean and the confidence limits (within 95%) for the familiarization session in the odor box. Subjects were first submitted to an infusion into the PMd with PBS (PBS group, n = 6) or atenolol 40 pmol (ATE 40 pmol group, n = 6), and, after 5 min, were microinjected with NMDA 100 pmol into the dlPAG paired with amyl acetate odor during 10 min in the conditioning session. CS1 represents the first-order CS exposure and CS2 represents the second-order context (no odor) exposure. *p<0.05 compared with the PBS group (repeated measures ANOVA followed by Newman-Keuls test).

Figure 8

doi: https://doi.org/10.1371/journal.pone.0050361.g008