Development of a Novel DNA Aptamer Ligand Targeting to Primary Cultured Tumor Endothelial Cells by a Cell-Based SELEX Method
Figure 7
In vitro tube formation assays to measure anti-angiogenesis activity of the AraHH001.
The tube formation was observed after 20 hours incubation with non-treated, zero-cycle library treated as a negative control, and AraHH001 treated mTECs on matrigel. A. Microscopic observation of tube formation of mTECs on matrigel. The tube formation was prohibited by AraHH001 treated mTECs on matrigel. Scale bar 100 µm. B. Quantitative analysis of tube formation. The tube length was calculated in pixels using one-way ANOVA after SNK test in each sample, and performed statistical analysis. Data are represented as mean± SD (n = 3 in each cases). **P<0.01 show significant differences NT and zero-cycle vs. AraHH001 treated group.