Quantitative Description of Glycan-Receptor Binding of Influenza A Virus H7 Hemagglutinin
Figure 1
Glycan receptor-binding specificity of FC, CC, NY/107 and mNY/107:A135T HA.
A, shows dose-dependent direct glycan array binding of FC HA. Specific high affinity binding to avian receptors (3′ SLN, 3′ SLNLN and 3′ SLNLNLN) and no binding to human receptors is observed. B, shows dose-dependent direct glycan array binding of CC HA. High affinity binding to avian receptors is observed. In comparison with FC, there is observable binding to human receptors (6′SLN-LN and 6′SLN) albeit at orders of magnitude lower affinity than binding to avian receptors. C, shows dose-dependent direct glycan array binding of NY/107 HA. High affinity binding to avian receptors (3′SLN-LN and 3′SLN-LN-LN) is observed with binding affinity for 3′SLN lower than that of FC and CC HAs. Binding to human receptor is observed but at much lower affinity (by orders of magnitude) than binding to avian receptors. D, shows dose-dependent direct glycan array binding of mutant mNY/107:A135T HA. Introduction of glycosylation sequon at Asn-133 does not seem to alter binding of this mutant HA in relation to the wild type.