The HER2-Binding Affibody Molecule (ZHER2∶342)2 Increases Radiosensitivity in SKBR-3 Cells
Figure 1
The binding trace of 125I-(ZHER2∶342)2 to SKBR-3 cells.
The interaction was monitored in real-time at room temperature using LigandTracer Grey. A) In the first experiment two concentrations of 125I-(ZHER2∶342)2 were added after each other. First, cells were incubated with 58 pM (ZHER2∶342)2 and thereafter, when equilibrium had been reached, more substance was added to a total concentration of 174 pM. B) In the second experiment, the off-rate after equilibrium of 174 nM exposure was followed for 24 hours. Data evaluation and estimation of the kinetic parameter KD were performed using the software TraceDrawer, using a one-to-one binding model with depletion correction. CPS (Counts per second).