Using Phage and Yeast Display to Select Hundreds of Monoclonal Antibodies: Application to Antigen 85, a Tuberculosis Biomarker
Figure 1
Strategy applied to the selection of antibodies using both phage and yeast display.
Antibodies are first selected against Ag85 using two rounds of phage display, after which the whole selection output is cloned into a yeast display vector using homologous recombination. A further one or two rounds of sorting by flow cytometry allow the subsequent isolation and testing of single clones.