Acute Ethanol Exposure Increases the Susceptibility of the Donor Hearts to Ischemia/Reperfusion Injury after Transplantation in Rats
Figure 4
Effect of acute ethanol exposure on DNA-strand breaks in cardiomyocytes.
Representative photomicrographs of (A) cardiomyocytes stained with cardiac troponin I (green) and nuclei with 4′, 6-diamidino-2-phenylindole (DAPI, blue), (B) nuclei with fragmented DNA visualized by TUNEL staining (red), and (C) merged image. Magnification ×400; scale bar: 20 µm. White arrows indicate TUNEL-positive cells (not all are marked) (D) Quantification of TUNEL-positive cells. We found DNA damage in the myocardium of rats treated with ethanol at 24 h. Eth indicates ethanol. *p<0.05 versus control.