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Human Breast Cancer Cells Are Redirected to Mammary Epithelial Cells upon Interaction with the Regenerating Mammary Gland Microenvironment In-Vivo

Figure 1

Human breast cancer cells differentiate in the mouse mammary gland.

Whole mounts of chimeric mammary gland outgrowths (A–D) were formed from the implantation of fragments from first generation chimeric mammary gland outgrowths (Figure S1A–D). Twelve weeks post implantation, these secondary chimeric mammary gland outgrowths were harvested, fixed in Carnoy's fixative, and stained overnight with Carmine Alum. A) Second generation whole mount of chimeric mammary gland outgrowth formed from implantation of a fragment from an original MDA-MB-468 chimera (1 K MDA-MB-468 human non-metastatic breast cancer cells plus 50 K primary mouse mammary epithelial cells); B) Second generation whole mount of chimeric mammary gland outgrowth formed from implantation of a fragment from an original MDA-MB-231-GFP chimera (10 K MDA-MB-231-GFP human metastatic breast cancer cells plus 50 K primary mouse mammary epithelial cells); C) Second generation whole mount of chimeric mammary gland outgrowth formed from implantation of a fragment from an original MDA-MB-231BRMS-GFP chimera (1 K MDA-MB-231BRMS-GFP metastasis-suppressed breast cancer cells plus 50 K primary mouse mammary epithelial cells); D) Second generation whole mount of chimeric mammary gland outgrowth formed from implantation of a fragment from an original hTERT-HME1 chimera (10 K hTERT-HME1 human mammary epithelial cells plus 50 K mouse mammary epithelial cells). Scale bars 1000 µm.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0049221.g001