Selective Histonedeacetylase Inhibitor M344 Intervenes in HIV-1 Latency through Increasing Histone Acetylation and Activation of NF-kappaB
Figure 7
M344 activates the HIV-1 LTR through induction of NF-κB.
(A) J-Lat clones A7 cells were transfected with HIV1-LTR luc, HIV1-LTRΔκB luc, HIV1-LTRΔAP-1luc, and HIV1-LTRΔSp1luc. At 24 hours posttransfection, the cells were treated or mock treated with M344 (200 nM) or TNF-α (10 ng/ml). Luciferase activity was measured after 24 hours of stimulation. The error bars indicate standard deviation. (B) J-Lat clones A7 cells were pretreated with various concentrations of (0, 2.5, 5 and 10 mM) aspirin for 3 hours and subsequently treated with M344 (100 nM) or TNF-α (10 ng/mL) or prostratin (100 nM) or control medium for 24 hours. The percentage of GFP+ cells (y-axis) in M344 or TNF-α stimulated cells in either the absence or the presence of the chemical inhibitors was measured by flow cytometry. Data represent the means±standard deviations of three independent experiments.