Culture Conditions Affect Cardiac Differentiation Potential of Human Pluripotent Stem Cells
Figure 5
The expression of neural markers was highest in hPSCs originating from Matrigel.
A) H7 cell line was hard to adapt on Matrigel combined with mTeSR1 medium. At first, the neural differentiation was observed primarily along the edges of the colonies on Matrigel in mTeSR1 medium. Finally, uneven neural rosette-like structures were formed in the colonies and the cell line was lost. B) Uneven neural rosette-like structures found in colonies of H7 cell line cultured on Matrigel in mTeSR1 medium stained with MAP-2. C) MAP-2 expressing neural-like cells and structures were found on Matrigel in mTeSR1 medium in all hPSC lines. Representative image of UTA.04602.WT cell line. D) MAP-2 expressing neural structures appeared also in END-2 co-cultures when hPSCs originated from Matrigel and mTeSR1 cultures with all hPSC lines. Representative image of H7 cell line. Scale bars, 200 μm. E) The highest amount of MAP-2 positive cells were found in Matrigel cultures. Scale bars, 200 μm. The expression of PAX-6 (F), Musashi (G) and Neurofilament (NF-68) (H) in END-2 co-cultures was significantly higher in cells originating from Matrigel and mTeSR1 medium than from MEF or SNL feeder cell layers almost in all time points. The data is collected from two individual differentiation experiments of H7, UTA.00112.hFF and UTA.04602.WT hPSC lines (n = 6 in all three conditions). Error bars show the standard error of the mean (SEM). ** p<0.01, * p<0.05.