Molecular Characterization of Chronic Lymphocytic Leukemia Patients with a High Number of Losses in 13q14
Figure 2
Quantitative RT-PCR validation for miR-15a, miR-29a, miR-155 and miR-223 in independent CLL patients.
Relative expression of miR-15a, miR-29a, miR-155 and miR-223 [represented as arbitrary units (a.u.)] was evaluated by individual TaqMan miRNA assays performed in duplicate and normalized to RNU43 (2−dCt). Box plots indicate the median value (horizontal line) and the 25th–75th percentile range (box) while whiskers showing the maximum and minimum values. Values outside this range are shown as outliers (open circles). P-values were determined by the Mann-Whitney U test. In every case, miRNAs downregulated in 13q-H CLL patients relative to 13q-L patients were also found to be downregulated by quantitative RT-PCR. Similar observations were made for miR-155, which was upregulated in 13q-H patients. All comparisons were statistically significant (P<0.05).