Urea Amidolyase (DUR1,2) Contributes to Virulence and Kidney Pathogenesis of Candida albicans
Figure 6
Effects of Dur1,2p expression in local inflammatory markers in infected kidneys.
(A) Concentrations of cytokines and chemokines in kidney extracts from C. albicans-infected mice at 1 to 5 days PI. Values at time 0 are mean values determined for sera from 5 control mice, and other data are mean ± standard deviation for 5 mice at each time point. Mice were infected iv using the wild type strain A72 or the dur1,2 mutant strain KWN6. Asterisks above an individual bar indicate that the serum value for that protein was significantly greater in mice infected with WT C. albicans than in mice infected with KWN6, * = p<0.05; ** = p<0.01; **** = p<0.0001. (B) Kidney iNOS expression in cellular infiltrates. Left panels represent WT infected kidneys, and right panels represent KWN6 infected kidneys. Upper panels show Candida colonization detected using GMS stain. Lower two panels show micrographs from the same area of an adjacent section stained using an antibody. Arrows indicate regions positive for iNOS (brown stain). Scale bar = 100 µm.