β-Adrenergic cAMP Signals Are Predominantly Regulated by Phosphodiesterase Type 4 in Cultured Adult Rat Aortic Smooth Muscle Cells
Figure 1
Expression analysis of mRNA encoding cAMP-PDE isoforms in RASMCs.
RT-qPCR reactions were carried out on mRNAs isolated from RASMCs. The expression of PDE1A (1A), PDE1B (1B), PDE1C (1C), PDE2A (2A), PDE3A (3A), PDE3B (3B), PDE4A (4A), PDE4B (4B), PDE4C (4C), PDE4D (4D), PDE7A (7A), PDE7B (7B), PDE8A (8A) and PDE8B (8B) was analyzed. A: PCR products were resolved by electrophoresis on a 3% agarose gel. Shown is the picture of a representative gel stained with GelRed®. Position of molecular weight markers is indicated in base pairs (Bp). B: mRNA expression was expressed using the 2ΔCt method as described in Materials and methods. Data are mean±SEM of 9 experiments. n.d. not detectable.