Apparent Defect in Yeast Bud-Site Selection Due to a Specific Failure to Splice the Pre-mRNA of a Regulator of Cell-Type-Specific Transcription
Figure 1
Budding-pattern phenotypes of wild-type and mutant strains.
Strains with the indicated genotypes were grown to exponential phase in SC medium and stained with Calcofluor to visualize bud scars. A montage of representative cells is shown for each strain. Strains used were (A) YEF473A; (B) YEF473; (C) STY254; (D) STY260; (E) KNY388; (F) STY627 (grown on 2% galactose +2% raffinose instead of glucose and at 30°C instead of 22°C); (G) STY484; (H) STY459; (I) STY550; (J) STY741; (K) STY742; (L) STY464.