A Culture-Independent Approach to Unravel Uncultured Bacteria and Functional Genes in a Complex Microbial Community
Figure 4
Bioluminescence of biosensor transducers incorporating DHN degradation genes.
ADPWH_Nag containing a nag2 gene cluster was rapidly activated to show bioluminescence after the addition of 50 µM DHN, confirming the function of the gene cluster. No bioluminescence was detected in the absence DHN. The negative control ADPWH_1274 did not respond to DHN. ADPWH_Nah containing a nah gene cluster was used as a positive control.