Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Offspring from Mouse Embryos Developed Using a Simple Incubator-Free Culture System with a Deoxidizing Agent

Figure 4

DeO2-WP culture system and development of full-term offspring.

(A, B) The culture dish was placed in a sealed plastic bag with a controlled O2 concentration, placed on a warm plate at 37°C and then covered with aluminum foil during the culture period. (C) Two-cell-stage embryos cultured for 24 h; (D) 8-cell-stage embryos cultured for 48 h and (E) morulae/blastocysts cultured for 72 h on the warm plate. Because the plastic bag was transparent, it was possible to observe embryos directly using an inverted microscope. (F) Mice derived from embryos cultured in the DeO2-WP culture system. (G) After two or three months, these offspring had grown to adulthood and randomly selected mice were proven fertile by natural mating.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0047512.g004