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Adhesion of Trypanosoma cruzi Trypomastigotes to Fibronectin or Laminin Modifies Tubulin and Paraflagellar Rod Protein Phosphorylation

Figure 2

Trypomastigote proteins modified by phosphorylation/dephosphorylation upon adhesion to laminin or fibronectin.

Incubation was for 2 h: (A) Phosphorylated proteins were stained with Pro-Q Diamond. (B) total protein profile developed with colloidal Coomassie blue staining; molecular mass markers (kDa) are shown on the ordinates; spots with variation in phosphorylation status are circled in red. (C) profile of phosphorylated and dephosphorylated spots upon 2 h incubation. (D) functional distribution of proteins from C.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0046767.g002