Improved Inhibitor Screening Experiments by Comparative Analysis of Simulated Enzyme Progress Curves
Figure 1
Simulated progress curves and differences between inhibited and uninhibited reactions.
(Left) Simulated progress curves for competitive (blue trace), uninhibited (black trace) reactions, and the difference (Δ[P]) between the two reactions (red trace). Δmax[P] is indicated by a dashed vertical line. (Right) Δ[P] between inhibited and uninhibited enzyme reactions for competitive (yellow), uncompetitive (green), non-competitive (orange), and mixed (blue) inhibition. In the simulation tool, progress curves for all types of inhibition are shown as in the left panel. Reaction parameters and variables can be entered and the results will be directly displayed in the graphs. Entered reaction conditions were: [S] = Km = 0.25Kmp = 10[I] = 400[Eo] = 100 µM; [Po] = 0 µM; kcat = 0.5 s−1; enzyme t(1/2) = 24 hours; Kic = Kiu = Ki-non = 5 µM for competitive, uncompetitive, and non-competitive inhibition; and Kiu = 5Kic = 15 µM for mixed inhibition.