SIRT1 Activators Suppress Inflammatory Responses through Promotion of p65 Deacetylation and Inhibition of NF-κB Activity
Figure 2
Development of a quantitative assay for measuring cellular acetylated p65 levels.
Flow diagram depicts the assay procedure, including BacMam p65 and BacMam p300 viral transduction, plating cells, compound treatment, cell lysis and detection of acetylated p65 protein by AlphaScreen format.