Role of Smad Proteins in Resistance to BMP-Induced Growth Inhibition in B-Cell Lymphoma
Figure 1
Normal and malignant B cells express BMP6 and BMP7.
BMP mRNA expression was determined by real-time RT-PCR, and the results are relative to the expression in human fetal brain tissue. (A) Centrocytes (CD19+CD38+IgD−CD77-) or centroblasts (CD19+CD38+IgD−CD77+) from normal human tonsils (relative expression ± SD of duplicates). (B) Lymphoma cell lines (means ± SEM, n = 3 for BMP6; n = 5 for BMP7). (C) Purified malignant B cells (CD20+CD10+Igκ/Igλ+) or tumor-infiltrating T cells (CD20−CD3+) from lymphoma patient samples (relative expression ± SD of duplicates). (D) Relative expression of BMP6 and BMP7 across non-Hodgkin’s lymphoma (NHL; left of line) and in normal B-cell populations (right of line) obtained from gene expression data set [16]. (E-F) Protein expression of BMP-7 was determined by Western blot analysis. Protein bands correspond to propeptide (lower band) and uncleaved full-length monomer (upper band). HeLa_BMP-7: Lysate from HeLa cells transfected with BMP-7. β-tubulin was used as loading control. One representative experiment (E) and mean values (F) of BMP-7 levels are shown (means ± SEM, n = 3).