Aquaporin-1 Translocation and Degradation Mediates the Water Transportation Mechanism of Acetazolamide
Figure 2
AZA induced CAs activity recovery and AQP1 reduction.
A, The time course of total CAs activity in rat kidney cortex. The carbonic anhydrase activity was assayed by endpoint colorimetric microtechnique after the treatment with AZA (40 mg/kg/day) or AZA (40 mg/kg/day) and NaHCO3 (30 mg/kg/day) at the indicated time. Results are expressed as a percentage of the control. Values are the means±S.E.M. *p<0.05, **p<0.01, ***p<0.001 compared to control. B and C, CAs and AQP1 extracted from rat kidney cortex were examined by immunoblotting in B and C. Each lane was loaded with 60 μg of total protein from rats at various times after AZA (40 mg/kg/day) (B) or combination with NaHCO3 (30 mg/kg/day) (C) treatment. The representative blotting images of CA II, CA IV and AQP1 are shown with β-actin as an internal control. Summary data is shown in down panels. Results are expressed as a percentage of the control. Values are the means±S.E.M. *p<0.05, **p<0.01 compared to control.