Detrimental Effects of RNAi: A Cautionary Note on Its Use in Drosophila Ageing Studies
Figure 1
Ubiquitous adult-induced expression of dsRNA targeting GFP for RNAi shortens lifespan in Drosophila females.
Mated female flies of the indicated genotype were fed 1 SYA food containing RU486 (red lines) or not (black lines) from day two of adulthood. A and B Log-rank test detected significant differences between the induced and uninduced conditions (p<0.0001, n = ∼100 per condition). C, D and E Log-rank test did not detected significant differences between the induced and uninduced conditions (p>0.05, n = ∼100 per condition).