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Effect of Remifentanil on Mitochondrial Oxygen Consumption of Cultured Human Hepatocytes

Figure 7

Modulation of the cytokine release.

HepG2 supernatants' IL-6 protein content after 24 h of incubation with medium alone (controls), TNF-α (10 ng/ml) or remifentanil (50 and 500 ng/ml) for 24 h or preincubation with remifentanil (50 and 500 ng/ml, 1 h incubation) followed by incubation with TNF-α (10 ng/ml) for an additional 24 h. In addition, cells were pretreated with naloxone at 1000 ng/ml followed by incubation with 50 or 500 ng/ml of remifentil or medium alone for an additional 24 hour (n≥3) (C: control, R50: remifentanil 50 ng/ml, R500: remifentanil 500 ng/ml). Data represent mean±SD. Statistical analysis for cytokine levels was performed by one-way analysis of variance (ANOVA): #p = 0.001. Afterwards all groups were divided into subgroups and subsequent one-way ANOVA was performed in the subgroups (subgroup 1: control, TNF-α, R50, R500 and naloxone (p = 0.003); subgroup 2: R50, R500, R50+naloxone, R500+naloxone p = 0.081; subgroup 3: TNF-α, R50+TNF-α, R500+TNF-α, p = 0.006) followed by an independent t-test in each subgroup. * = independent t-test, TNF-α vs. control (p = 0.032). & = independent t-test R500+TNF-α vs. TNF-α (p = 0.008).

Figure 7

doi: https://doi.org/10.1371/journal.pone.0045195.g007