Dynamic Link between Histone H3 Acetylation and an Increase in the Functional Characteristics of Human ESC/iPSC-Derived Cardiomyocytes
Figure 7
TSA enhances electrophysiological function in hiPSC-CMs accompanied by histone H3 acetylation.
(A) Western blot analyses show increased histone H3 acetylation levels in hiPSC-CMs treated with 100 nM TSA for 48 hrs. (B) Results from western blot analyses comparing Sus to Ad cultures (n = 7) and TSA to DMSO treatments (n = 9) show increased AcH3 levels in hiPSC-CMs. (C) Sequential MEA analyses in all hiPSC-CM colonies on Day 2 and Day 6 showed that TSA altered the electrophysiological phenotypes evaluated on the basis of their sensitivity to 200 nM of E4031 (n = 8). (D) Sequential MEA analyses in all hiPSC-CM spheroid on Days 2, 6 and 9 after plating showed electrophysiological phenotypes improved reproducibly following 2 days of TSA treatment in the presence of 200 nM E4031 (n = 7 for DMSO, n = 8 for TSA). Changes in relative FPD values (Day 2 = 1) from Day 2 to Days 6 and 9 and the mean±SD are shown. †, tendency to differ (p<0.1) only on Day 6.