Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Differential Requirements in Endocytic Trafficking for Penetration of Dengue Virus

Figure 3

Transport of DENV particles to early endosomes.

A. Cells transiently transfected with the GFP-tagged versions of Rab5 wt and S34N, and the plasmid pGFP-C1 were infected with DENV-1 HW, DENV-2 NGC or DENV-2 16681 (MOI:10 PFU/cell). After 1 h of infection, cells were fixed and processed to visualize GFP transgene expression and internalized viral particles by immunofluorescence staining using mouse anti-E glycoprotein antibody and TRITC-labelled anti-mouse IgG. B. Cells transfected as in A) were infected with DENV-1 HW, DENV-2 NGC or 16681 (MOI:1 PFU/cell). After 24 h of infection cultures were fixed and immunofluorescence staining was performed as in A. C. For quantification of samples shown in B, 250 transfected cells with similar levels of GFP expression were screened and cells positive for viral antigen were scored. D. Cells transfected as in A were then incubated with TRITC-labelled transferrin during 30 min. Then, cells were fixed and fluorescence was visualized.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0044835.g003