Graft versus Host Disease in the Bone Marrow, Liver and Thymus Humanized Mouse Model
Figure 3
Characterization of T cells in BLT GVHD mice.
(A) IFNγ ELISA on supernatants from skin explants cultured for 12 hours ex vivo. p = .0001 by a two-tailed Student's t-test. (B) Realtime PCR analysis for the indicated genes on RNA samples isolated from the skin of control and BLT GVHD mice. “**” indicates that the signal for both HPRT and the gene of interest were below the limit of detection. (C) Realtime PCR analysis for the indicated genes on RNA samples isolated from the skin of control and BLT GVHD mice. For all genes displayed, p<.05 by a two-tailed Student's t-test. (C) Flow cytometric analysis of cells isolated from the skin of BLT GVHD and control mice and stained for the indicated markers. (D) ELISA for the indicated cytokines on tissue culture supernatants of CD4+ T cells sorted from control and BLT GVHD skin as in (C) and restimulated with PMA and ionomycin. For IFNγ, P<.0001, for IL17, P = .0027, both by a two-tailed Student's t-test. (E) IFNγ ELISA on the tissue culture supernatants of CD4+ T cells isolated from the spleen of control and BLT GVHD mice by magnetic bead sorting and restimulated with PMA + ionomycin. Comparing stimulated healthy and GVHD T cells, P = .0016 by a two-tailed Student's t-test.