RBMS3 at 3p24 Inhibits Nasopharyngeal Carcinoma Development via Inhibiting Cell Proliferation, Angiogenesis, and Inducing Apoptosis
Figure 4
Ectopic expression of RBMS3 induces apoptosis.
(A) Representative images of TUNEL staining. After STS treatment, more apoptotic cells (green) were detected in SUNE1-RBMS3 cells compared to SUNE1-V1 cells (arrowhead). The apoptotic indexes of SUNE1-RBMS3 cells and SUNE1-V1 cells (before and after STS treatment) were summarized in the right panel (*p<0.05). (B) This event was measured by loss of mitochondrial ΔΨm using JC-1 dye. Red or orange fluorescence indicates intact mitochondria, whereas green fluorescence indicated a collapse in mitochondrial ΔΨm. Prior to STS treatment the SUNE1-RBMS3 cells reveal a significant ΔΨm loss (green fluorescence, ×400) and higher apoptotic index compared to SUNE1-V1 cells (red/orange fluorescence; left panel) (*p<0.05). (C) The cleavages of caspase-9, caspase-8, and PARP were compared between RBMS3-transfected and empty vector-transfected SUNE1 cells. β-actin was used as loading control.