Novel Small Noncoding RNAs in Mouse Spermatozoa, Zygotes and Early Embryos
Figure 6
Search for double-stranded piRNA precursors of spR-12 and -13 by strand-specific RT-PCR.
Total RNA from adult mouse testes and epididymis was reverse-transcribed with a primer with an antisense sequence, with a primer with a sense sequence, and with no primer. PCR was conducted using gene-specific primers (Table 5). The product was 301 bp for the spR-12 locus, and 351 bp for the spR-13 locus, whose nucleotide sequences are shown in Figure 7. LINE 1 and β-actin were used as positive and negative controls, respectively.