Epidermal Growth Factor Receptor Tyrosine Kinase Defines Critical Prognostic Genes of Stage I Lung Adenocarcinoma
Figure 1
Diagrams of experimental procedures and gene set selection by SSM analysis.
(A) A diagram of the in vivo experimental procedures. The serum-starved cells were stimulated with EGF (100 ng/mL) in the presence or absence of gefitinib (0.5 μM) for 48 h at 37°C. Before stimulation with EGF, cells were starved for 24 h at 37°C. Total RNA was isolated at each time point as indicated by the arrows (19 time points); the same experiments were performed two or three times at several time points. (B) Schematic view of time-course gene expression patterns of predicted or observed gene expression levels. The blue solid line represents a predicted gene expression pattern based on the EGF-response SSM, using the observed gene expression levels derived from the EGF-treated cells (x). (C) The red solid line represents a predicted gene expression pattern based on the EGF-response SSM, using the observed gene expression levels derived from the EGF+gefitinib-treated cells (o). (D, E) A representative gene expression pattern of gefitinib-sensitive genes (D) and -insensitive genes (E). Left panels: observed gene expression patterns in EGF-treated cells (x in blue) and EGF-response SSM-predicted gene expression patterns (blue dotted line). Right panels: observed gene expression patterns in EGF+gefitinib-treated cells (o in red) and the EGF-response SSM-predicted gene expression patterns (red solid line).