KCa3.1 and TRPM7 Channels at the Uropod Regulate Migration of Activated Human T Cells
Figure 2
Movement of human activated CD3+ T cells on ICAM-1 surfaces by random walk.
A. Representative time-lapse bright-field microscopy of an activated T cell migrating on an ICAM-1 surface. Asterisks indicate the starting point of the cell of interest, and the arrow shows the direction of the movement. The dotted line represents the coming track whereas the continuous line is the covered distance. Scale bar = 5 µm. B. Time-dependent distribution of the migration velocity of two representative activated T cells each defined by either a continuous or dotted line. C. Tracks of 54 migrating T cells on ICAM-1 normalized to their starting coordinates. The x-y coordinates were determined in MetaMorph software. The final x–y values reached by 5 cells are out of the x/y axis ranges. The corresponding x–y coordinates are given for each cell at the end of the extended dotted lines.