Supplementing with Non-Glycoside Hydrolase Proteins Enhances Enzymatic Deconstruction of Plant Biomass
Figure 5
Thermostability of CbXyn10A (A, B, and C) and hydrolysis of Miscanthus by CbXyn10A (D, E, and F).
A–C: Thermostability of CbXyn10A in the presence of CbHsp18 (A), MkHistone1 (B), and RNase A (C). One micromolar of CbXyn10A was incubated with 8 µM, 16 µM, or 32 µM of CbHsp18, MkHistone1, or RNase A in a pH 6.0 citrate buffer in a total volume of 500 µl. The mixtures were shaken end-over-end at 70°C for 24 hr. D–E: 1 µM of CbXyn10A was incubated with 20 mg/ml Miscanthus in the absence or presence of 8 µM, 16 µM, and 32 µM each of CbHsp18 (D), MkHistone1 (E), and RNase A (F) in a pH 6.0 citrate buffer in a total volume of 500 µl. The reaction mixtures were rotated end-over-end at 70°C for 24 hr.